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When cellulolytic activity of CAA_GH5 was assayed through Congo red method by measuring the size of halo zone, the recombinant Bacillus subtilis RIK1285 expressing CAA_GH5 showed a comparable cellulolytic activity to B. subtilis RIK1285 expressing Cel5, a

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Congo is the world's leading producer of cobalt, supplying more than 60 percent of the metal to the world. In recent years, demand for cobalt, a primary component in batteries for electric cars has risen sharply. The new mining code will likely lead to high costs for consumers.

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As Congo Red only binds to long chain polysaccharides but not short chain, the short chain therefore are washed off during staining procedure resulting in halo formation . The results are shown in Fig. 3. Fig.3 Activity determination of YebF-CenA using Congo Red assay. Left T7-RBS-yebF-cenA medium supernatant and control (LB liquid medium).

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